A developed thin-layer chromatography method for the determination of genistein-7-O-[α-rhamnopyranosyl-(1 → 6)]-β-glucopyranoside in Derris scandens

Abstract

A simple thin-layer chromatography (TLC)–densitometric and a TLC–image analysis method were developed for the quantitative determination of an active ingredient, genistein-7-O-[α-rhamnopyranosyl-(1 → 6)]-β-glucopyranoside (GTG) in Derris scandens, by using GTG and genistein as reference standards. Due to a large polarity difference between GTG and genistein, chromatographic analysis was achieved on a silica gel 60 F254 TLC plate using a multiple development technique, with ethyl acetate–methanol–water (8:1.3:1, V/V) and n-hexane–ethyl acetate (6:4, V/V) as the first and the second mobile phase, respectively. The method was validated and applied for the determination of GTG in D. scandens. The analytical results obtained from the TLC–densitometric, TLC–image analysis and HPLC methods were compared and found to be not significantly different.