A deuterium tracer experiment for simulating accumulation and elimination of organically bound tritium in an edible flatfish, olive flounder

Abstract

Tritium (3H, T) is discharged by nuclear facilities into coastal oceans as tritiated water (HTO). When the concentration of HTO in seawater increases, the accumulation of organically bound tritium (OBT) in edible fish becomes a concern because of its longer residence time than HTO. To evaluate the accumulation potential of OBT in olive flounder (Paralichthys olivaceus), a commercially important edible fish in northeast Asia, we experimentally exposed the fish to seawater enriched with deuterium (2H, D) as a substitute for tritium. Progressive increases and decreases in the concentration of organically bound deuterium (OBD) were observed in the edible part (i.e., muscle) of the fish during the period of exposure to 2H (161 days) and the subsequent period of elimination of OBD (196 days). The measured concentration of OBD was analyzed using a newly developed single-compartment model to describe the metabolism of OBD in muscle via the following three transfer pathways: formation of OBD from 2H in water, elimination of OBD by catabolism, and ingestion of feed with natural abundance of OBD. The model estimates were in good agreement with the measured muscle OBD concentrations. The formation and elimination rate constants for OBD in the muscle were estimated by fitting our model to the measured data. The biological half-life of OBT in the muscle, estimated from the elimination rate constant, was 133 days, which was far longer than that of HTO in the free water of the muscle. Our model facilitates the estimation of OBT accumulation potential in olive flounder inhabiting coastal areas near nuclear facilities, and thus, will help to assess the radiation dose that humans are exposed to from ingesting seafood.