Abstract
BackgroundFeline immunodeficiency virus (FIV) is a lentivirus associated with AIDS-like illnesses in cats and has been used as a model for the study of human immunodeficiency virus (HIV). A feature of HIV and FIV infection is the continually increasing divergence among viral isolates between different individuals, as well as within the same individuals.Methodology/Principal FindingsThe goal of this study was to determine the phylogenetic patterns of viral isolates obtained within the United States (U.S.) by focusing on the variable, V3-V4, region of the FIV envelope gene.Conclusions/SignificanceData indicate that FIV, from within the U.S., localize to four viral clades, A, B, C, and F. Also shown is the geographic isolation of strains where clade A and clade B are found predominately on the west coast; however, clade B is also found throughout the U.S. and represents the predominant clade. This study presents a complete and conclusive analysis of FIV isolates from within the U.S. and may be used as the essential basis for the development of an effective multi-clade vaccine.
Highlights
Feline immunodeficiency virus was first isolated from a cat exhibiting an immunodeficiency-like syndrome [1]
Due to the similarities in Feline immunodeficiency virus (FIV) and human immunodeficiency virus (HIV) pathogenesis, fiv has become as the only small animal non-primate model for the study of HIV disease, therapy, and prophylaxis
Genomic DNA that contained the FIV proviral DNA was isolated from the lymphocytes using a mammalian genomic DNA Isolation miniprep kit
Summary
Feline immunodeficiency virus was first isolated from a cat exhibiting an immunodeficiency-like syndrome [1]. This isolate from petaluma, california was designated the petaluma strain, and has become one of the most common laboratory strains used for research investigating lentiviral pathogenesis, antiviral chemotherapy design and testing, and for developing vaccine strategies as a small animal model for HIV [2,3,4,5,6,7]. Feline immunodeficiency virus (FIV) is a lentivirus associated with AIDS-like illnesses in cats and has been used as a model for the study of human immunodeficiency virus (HIV). A feature of HIV and FIV infection is the continually increasing divergence among viral isolates between different individuals, as well as within the same individuals
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