Abstract
The present study explores Countercurrent Separation (CCS) as a powerful method to Deplete and Enrich Select Ingredients to Generate Normalized Extract Resources. A DESIGNER extract of European licorice (Glycyrrhiza glabra L.) depleted of glabridin (1) and four congeneric metabolites (2 – 5) was prepared for biological testing. Solvent system (SS) selection was based on partition coefficient (K value) determination measured by UV following two-phase distribution. The SS composed of Hexanes/Ethyl Acetate/Methanol/Water 7:5:6:4, v/v, was used with the lower phase mobile on a High Speed Countercurrent Chromatography (HSCCC) apparatus for the fractionation of G. glabra crude extract. An orthogonal CCS step was developed for the continued purification of each compound. The purity and identity of each compound was determined by qHNMR. All other extract resources were recombined to build up the DESIGNER extract. The results obtained highlight that CS enables the simultaneous and targeted enrichment and depletion of structurally related metabolites.
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