Abstract
A 2.3 kb single-copy genomic EcoRI-BamHI fragment was isolated from the putative first intron of the human BCR gene and subcloned into the Bluescribe vector pBS M13+ (pA-EB2.3). Multiple enzymes resolve a two-allele deletion/insertion type RFLP. A 1 kb difference between the alleles is seen in BamHI, Bg1II and HindIII digests. The deleted/inserted material includes EcoRI and TaqI sites; thus, using these enzymes the fragment lengths corresponding to the alleles do not differ by 1 kb. The BCR gene is within chromosome band 22q11. A family was studied in which the parents represented both homozygotes. The child was heterozygous as expected.
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