Abstract
The development of double haploids (DHs) is a straightforward path for obtaining pure lines but has multiple bottlenecks. Among them is the determination of the optimal stage of pollen induction for androgenesis. In this work, we developed Microscan, a deep learning-based system for the detection and recognition of the stages of pollen development. In a first experiment, the algorithm was developed adapting the RetinaNet predictive model using microspores of different eggplant accessions as samples. A mean average precision of 86.30% was obtained. In a second experiment, the anther range to be cultivated in vitro was determined in three eggplant genotypes by applying the Microscan system. Subsequently, they were cultivated following two different androgenesis protocols (Cb and E6). The response was only observed in the anther size range predicted by Microscan, obtaining the best results with the E6 protocol. The plants obtained were characterized by flow cytometry and with the Single Primer Enrichment Technology high-throughput genotyping platform, obtaining a high rate of confirmed haploid and double haploid plants. Microscan has been revealed as a tool for the high-throughput efficient analysis of microspore samples, as it has been exemplified in eggplant by providing an increase in the yield of DHs production.
Highlights
The development of commercial hybrids from elite materials and the introgression of genes of interest from related species that allow expanding the genetic background of crops [1] are basic tools for breeders [2]
Microscan has been revealed as a tool for the high-throughput efficient analysis of microspore samples, as it has been exemplified in eggplant by providing an increase in the yield of double haploids (DHs) production
The precision success percentages were in all cases higher than 80%, the highest being in the case of mature pollen where a value of 92.19% was reached, followed by the vacuolated microspores, which reached
Summary
The development of commercial hybrids from elite materials and the introgression of genes of interest from related species that allow expanding the genetic background of crops [1] are basic tools for breeders [2]. To obtain genetically uniform F1 hybrid plants, the development of pure lines (highly homozygous organisms that breed true by selfing) is one of the main objectives of plant breeders. The development of pure lines is not simple and may require a long time [4]. The conventional method to obtain lines has traditionally been the application of successive rounds of self-fertilization and selection, generally requiring 7 to 10 generations for achieving a high degree of homozygosity. By reprogramming the gametophytic development pathways to sporophytic or embryogenic development pathways through the application of different inducing
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
