A decrease of cyclophosphane toxic effect by the combined action with amino acids in the tissue culture of liver

Abstract

In organotypic culture investigated the combined effect iproto-like agent cyclophosphamide and the encoded L-amino acids on the development of the processes of proliferation and apoptosis in explants of the liver tissue of Mature rats. Various amino acids, in a concentration of 0.05 ng/ml had either a stimulating or a depressing effect on the cellular proliferation in the growth zone of explants. When introduced into the culture medium of cyclophosphamide at a concentration of 1 mg/ ml was inhibition of cell proliferation due to the development of apoptosis, verified with immunocytochemical detection of enhanced expression of protein p53. Coadministration of cyclophosphamide in conjunction with each of the amino acids led to a significant decrease in the inhibitory effect of cyclophosphamide. Amino acids with charged side radical, acid from glutamic acid and arginine at a major – was able to completely eliminate the inhibitory effect of cyclophosphamide in organotypic tissue culture of rat liver. Amino acids with charged side chains can be considered as the simplest of the regulators and stimulants of physiological functions. We know that of all the physico-chemical properties of amino acids is the hydrophobicity of the side groups are important for their intermolecular interactions. Mechanisms of action of these amino acids, the cells can have universal features, which is consistent with the assumption about the participation of electrostatic and hydrophobic interactions in these processes. Thus, L amino acids can have a protective effect on the cell proliferation of the liver tissue from the toxic action iproto-like agents.