Abstract
A Decellularized Human Corneal Scaffold for Anterior Corneal Surface Reconstruction
Highlights
Decellularized by various concentrations of sodium deoxycholate (SD) with deoxyribonuclease I in comparison to the normal human cornea (NHC) using hematoxylin and eosin (H&E) staining
Human DM− corneas decellularized with different concentrations of standard deviation (SD) were initially screened by hematoxylin and eosin (H&E) staining, immunofluorescent staining for human leukocyte antigen (HLA)-ABC was used to assess the presence of residual cellular/membranous material, and nuclear staining with 4′,6-diamidino-2-phenylindole (DAPI) (Fig. 1A)
The use of 0.5% SD did not result in complete removal of cellular debris as apparent in H&E (Fig. 1A, higher magnification, arrowheads) and HLA-ABC staining’s (Fig. 1A, arrows)
Summary
Decellularized by various concentrations of sodium deoxycholate (SD) with deoxyribonuclease I in comparison to the normal human cornea (NHC) using hematoxylin and eosin (H&E) staining. The higher magnification images of H&E staining showing the stromal cells in NHC (arrowheads) and cellular debris in 0.5% SD DAPI staining reveals nuclei in NHC without remaining nuclei/nuclear debris in DHC.
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