Abstract
Alkyne functional groups have Raman signatures in a region (1800 cm−1 to 2800 cm−1) that is free from interference from cell components, known as the “silent region”, and alkyne signals in this region were first utilized a decade ago to visualize the nuclear localization of a thymidine analogue EdU. Since then, the strategy of Raman imaging of biological samples by using alkyne functional groups, called alkyne-tag Raman imaging (ATRI), has become widely used. This article reviews the applications of ATRI in biological samples ranging from organelles to whole animal models, and briefly discusses the prospects for this technique.
Highlights
Subha Bakthavatsalam did herPhD under the supervision of DrAnkona Datta at Tata Institute of Fundamental Research, Mumbai, India
We showed that deuterated g-linoleic acid (D-GLA) is selectively cytotoxic to tumor cells over normal cells
We demonstrated the applicability of alkyne tag Raman imaging (ATRI) to lipid imaging by developing analogues of CoQ, which is an essential and abundant mitochondrial lipid
Summary
Subha Bakthavatsalam synthetic organic chemistry lab at RIKEN, Wako, Japan. Her current research interests include developing Raman based probes for imaging biomolecules. After the postdoctoral training at RIKEN, he was appointed as an assistant professor at the University of Tokyo in 2007. He returned to RIKEN as a research scientist and a group leader in the ERATO Sodeoka. Fluorescence microscopy is the most widely used imaging technique for the study of biological samples in an academic setting. Fluorescence imaging requires labelling of the target biomolecules either with fluorescent small molecules or with genetically encoded fluorescent proteins. Some of the modes used for biological imaging are discussed in brief
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