A Dataset of Experimental HLA‐B*2705 Peptide Binding Affinities

Abstract

T‐cell epitopes form the basis of many vaccines, diagnostics, and reagents. Current methods for the in silico identification of T‐cell epitopes rely, in the main, on the accurate quantitative prediction of peptide‐Major Histocompatibility Complex (pMHC) affinity using data‐driven computational approaches. Here, we describe a dataset of experimentally determined pMHC binding affinities for the problematic human class I allele HLA‐B*2705. Using an in‐house, FACS‐based, MHC stabilization assay, we measured binding of 223 peptides. This dataset includes both nonbinding and binding peptides, with measured affinities (expressed as −log10 of the half‐maximal binding level) ranging from 1.2 to 7.4. This dataset should provide a useful independent benchmark for new and existing methods for predicting peptide binding to HLA‐B*2705.