Abstract
Pathological hypertrophy underlies sudden cardiac death due to its high incidence of occurrence of ventricular arrhythmias. The alteration of transmural electrophysiological properties in hypertrophic cardiac murine tissue has never been explored previously. In this dataset, we have for the first time conducted high-throughput simultaneous optical imaging of transmembrane potential and calcium transients (CaT) throughout the entire hypertrophic murine hearts at high temporal and spatial resolution. Using ElectroMap, we have conducted multiple parameters analysis including action potential duration/calcium transient duration, conduction velocity, alternans and diastolic interval. Voltage-calcium latency was measured as time difference between action potential and CaT peak. The dataset therefore provides the first high spatial resolution transmural electrophysiological profiling of the murine heart, allowing interrogation of mechanisms driving ventricular arrhythmias associated with pathological hypertrophy. The dataset allows for further reuse and detailed analyses of geometrical, topological and functional analyses and reconstruction of 2-dimensional and 3-dimentional models.
Highlights
Background & SummarySudden cardiac death (SCD) due to ventricular arrhythmias causes 3.7 million deaths worldwide every year[1]
SCD is highly associated with pathological hypertrophy though it occurs in a broad spectrum of other cardiac conditions
Electrophysiological studies over the past few decades in animal models were largely based on the isolated single cell preparations. These studies enabled us to gain a better understanding of how pathological cardiac remodelling may predispose to ventricular arrhythmias that may lead to SCD
Summary
Sudden cardiac death (SCD) due to ventricular arrhythmias causes 3.7 million deaths worldwide every year[1]. This allows profiling of the ventricular transmural and regional gradients in Vm and CaT and characterization of the transmural and regional profiles of action potential and CaT alternans associated with ventricular arrhythmias Such high-throughput transverse slice optical imaging technique provides a unique opportunity for exploring the mechanistic association of transmural electrical and intracellular Ca2+ remodelling in pathological hypertrophy and ventricular arrhythmogenesis that leads to SCD. The approach provides a basis for further use of drug testing and screening and for developing 2D and 3D computational models in the future
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
