Abstract
D-lactate dehydrogenase (D-LDH) converts D-lactate (the end product of glyoxalase system) to pyruvate and thereby completes the detoxification process of methylglyoxal. D-LDH detoxifies and diverts the stress induced toxic metabolites, MG and D-lactate, towards energy production and thus, protects the cell from their deteriorating effects. In this study, a D-LDH enzyme from rice (OsD-LDH2, encoded by Os07g08950.1) was characterized for its role in abiotic stress tolerance. For this, a combination of in silico, molecular, genetic and biochemical approaches was used. The kinetic analysis revealed OsD-LDH2 to be the most efficient D-LDH enzyme in comparison to D-LDHs from other plant species. Heterologous overexpression of OsD-LDH2 provides tolerance against multiple abiotic stresses in E. coli, yeast and plant system. The analysis of D-LDH mutant and OsD-LDH2 overexpressing transgenic plants uncovered the crucial role of D-LDH in mitigation of abiotic stresses. OsD-LDH2 overexpressing plants maintained lower level of ROS and other toxic metabolites along with better functioning of antioxidant system. This is the first report on correlation of D-LDH with multiple abiotic stress tolerance. Overall, OsD-LDH2 emerged as a promising candidate which can open a new direction for engineering stress tolerant crop varieties by maintaining their growth and yield in unfavorable conditions.
Highlights
D-lactate dehydrogenase (D-LDH) converts D-lactate to pyruvate and thereby completes the detoxification process of methylglyoxal
The present study identified OsD-LDH2 as a major role player in providing multiple abiotic stress tolerance
The Arabidopsis thaliana seeds of mutant (M), wildtype (WT) and OsD-LDH2 overexpressing transgenic plants (D2TG) were sown on half strength MS agar plates supplemented with different concentrations of various stresses such as D-lactate (0–10 mM), Methylglyoxal (0–1 mM), NaCl (0–150 mM), Mannitol (0–150 mM) and H2O2 (0–5 mM)
Summary
D-lactate dehydrogenase (D-LDH) converts D-lactate (the end product of glyoxalase system) to pyruvate and thereby completes the detoxification process of methylglyoxal. The heterologous overexpression of OsD-LDH2 provided tolerance against multiple abiotic stresses in E. coli, yeast and model plant Arabidopsis. The Arabidopsis thaliana seeds of mutant (M), wildtype (WT) and OsD-LDH2 overexpressing transgenic plants (D2TG) were sown on half strength MS agar plates supplemented with different concentrations of various stresses such as D-lactate (0–10 mM), Methylglyoxal (0–1 mM), NaCl (0–150 mM), Mannitol (0–150 mM) and H2O2 (0–5 mM).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
