Abstract
The Aglaia genus belong to Meliceae family is unique plant species because the presence of rocaglate and rocaglamide which is so far isolated only from Aglaia genus, indicate that type of this compound as a chemical marker for the genus of Aglaia. This type of compound known to have strong activity, such as insecticide and cytotoxic. This study describe the isolation, structure elucidation, and cytotoxic activity of an isolated rocaglate compound. Dried stembark of A. argentea extracted with methanol and partition between n-hexane, ethyl acetate, and n-butanol, respectively The extracts were tested against P-388 murine leukemia cells and the ethyl acetat showed strongest activity with IC50 value of 15.5 mg/mL. The ethyl acetate then was separated and purified with chromatography technique to obtain isolated compound 1. The chemical structure of isolated compounds were elucidated by spectroscopic methods including one and two-dimensional NMR as well as high-resolution mass spectrometric analysis and identified as a methyl rocaglate. Compound 1 showed strong cytotoxic activity with an IC50 value of < 0.1 μg/mL.
Highlights
Aglaia is the largest genus belong to Meliaceae family contain more than 150 species (Su et al, 2006; Awang et al, 2012) and about 65 species grown in Indonesia (Wood, Silverstain & Nakajima, 1970)
During the course of our continuing search for anticancer candidate compounds from Indonesia Aglaia plants, we isolated and described cytotoxic triterpenoids from the bark of A. smithii and A. eximia (Harneti et al, 2012; 2014) as well as a lignan and bisamides from the bark of A. eximia (Sianturi et al, 2015; 2016)
Species, we found that ethyl acetate extracts of the bark of A. argentea exhibited a significant cytotoxic activity against P-388 murine leukemia cells with IC50 value of 15.48 ± 0.03 g/mL
Summary
Aglaia is the largest genus belong to Meliaceae family contain more than 150 species (Su et al, 2006; Awang et al, 2012) and about 65 species grown in Indonesia (Wood, Silverstain & Nakajima, 1970). Species, we found that ethyl acetate extracts of the bark of A. argentea exhibited a significant cytotoxic activity against P-388 murine leukemia cells with IC50 value of 15.48 ± 0.03 g/mL. Column chromatography was conducted on silica gel 60 (Kanto Chemical Co., Inc., Japan) and (Merck, Darmstadt, Germany). Fraction D (1.12 g) was chromatographed on a Medium Presure Liquid Chromatography (MPLC) column of silica gel, eluted with CHCl3–EtOAc (50:1), to give eight subfractions (D01–D08). IC50 values were taken from the plotted graph of percentage live cells compared to control (%), receiving only PBS and DMSO, versus the tested concentration of compounds (μg/mL).
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