Abstract
In this study, one triterpenoid compound from n-hexane fraction of Lantana camaraLinn leaves has cytotoxic activity was isolated. Isolation was carried out using gravity chromatographycolumnand purification by recrystallization method. Isolated compound was obtained white solid with melting point 252-253oC. The structure of isolated compound was elucidated using spectroscopic analysis Ultraviolet (UV), Infrared (IR), 1H-Nuclear Magnetic Resonance (1H-NMR), 13C-Nuclear Magnetic Resonance (13C-NMR) Heteronuclear Multiple Bond Connectivity (HMBC), Heteronuclear Multiple Quantum Correlation (HMQC), Distortionless Enhancement Polarization Transfer (DEPT) and comparative literature data, identified as Lantadene A (22β-angeloyloxy-3-oxoolean-12-en-28-oic-acid) with molecule formula C35H52O5. The Lantadene A compound was evaluated for cytotoxic activity against shrimp larvae Artemia SalinaLeach using Brine Shrimp Lethality Test(BSLT) method, showed strong cytotoxic activity with an LC50value of 48.97 µg/mL.
Highlights
Several compounds have been isolated from Lantana camara Linn such as 9-Hydroxy-lantadene A (M.Z, Suryati & Efdi, 2018), 24-Hydroxy-lantadene B, 24Hydroxy-lantadene X, 24-Hydroxy-lantadene D, 22Hydroxy-4-epi-hederagonic acid, 3β-Hydroxy-lantadene C (Abdjul, et al, 2017), ursolic acid (Jamal, Amir, Ali, & Mujeeb, 2018), oleanolic acid (Vyas & Argal, 2014), camaric acid, Lantadene C, camarinic acid, camaraside, 24-Hydroxy-3-oxours-12-en-28-oic acid (Kumar, et al, 2016)
This paper reported the isolation of triterpenoid compound from n-hexane fraction of Lantana camara Linn leaves and its cytotoxic activity using Brine Shrimp Lethality Test (BSLT) method
The isolated compound was carried out the melting point measurement and the structure was elucidated using spectroscopic analysis UV, IR, and Nuclear Magnetic Resonance (NMR) (1D and 2D)
Summary
Lantana camara Linn plant has various activities such as antibacterial (Ganjewala, Sam, & Khan, 2009), anticancer (Pour, Latha & Sashidaran, 2011), antifungal (Passos et al, 2012), anti-inflamatory, antiviral, antitubercular, nematicidal, hepatotoxic, antimutagenic (Kumar, Katiyar, Singh, Surender & Tarun, 2016), antioxidant (Suryati, Santoni, M.Z, & Aziz, 2016), antidiabetic (Kazmi et al, 2012), and cytotoxic activity (Ediruslan, Manjang, Suryati & Aziz, 2015).Several compounds have been isolated from Lantana camara Linn such as 9-Hydroxy-lantadene A (M.Z, Suryati & Efdi, 2018), 24-Hydroxy-lantadene B, 24Hydroxy-lantadene X, 24-Hydroxy-lantadene D, 22Hydroxy-4-epi-hederagonic acid, 3β-Hydroxy-lantadene C (Abdjul, et al, 2017), ursolic acid (Jamal, Amir, Ali, & Mujeeb, 2018), oleanolic acid (Vyas & Argal, 2014), camaric acid, Lantadene C, camarinic acid, camaraside, 24-Hydroxy-3-oxours-12-en-28-oic acid (Kumar, et al, 2016).Previous research, Ediruslan et al (2015) has reported the cytotoxic of n-hexane, and ethyl acetate fractions of Lantana camara Linn leaves. n-Hexane and ethyl acetate fractions showed strong toxicity with LC50 values of 34 and 27 μg/mL, respectively. Keywords : Lantana camara Linn, Lantadene A, Artemia Salina Leach, Cytotoxic. Ediruslan et al (2015) has reported the cytotoxic of n-hexane, and ethyl acetate fractions of Lantana camara Linn leaves. Ediruslan et al (2015) has reported triterpenoid compound (Lantanilic acid) show strong cytotoxic potential with LC50 values of 27.99 μg/mL of ethyl acetate, but not yet reported cytotoxic compound from n-hexane fraction of Lantana camara Linn leaves.
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