Abstract
Pulp regeneration is to replace the inflamed/necrotic pulp tissue with regenerated pulp-like tissue to rejuvenate the teeth. Self-assembling peptide hydrogels RADA16-I (Ac-(RADA16-I)4-CONH2) can provide a three-dimensional environment for cells. The stem cell factor (SCF) plays a crucial role in homing stem cells. Combining these advantages, our study investigated the effects of SCF-RADA16-I on adhesion, proliferation, and migration of human dental pulp stem cells (DPSCs) and the angiogenesis of human umbilical vein endothelial cells (HUVECs). The β-sheet and grid structure were observed by circular dichroism (CD), scanning electron microscopy (SEM), and atomic force microscopy (AFM). Cytoskeleton staining, living cell staining, cell viability, cell migration, angiogenesis, and western blot assays were performed, and the results indicated that all the SCF groups were superior to the corresponding non-SCF groups in cell adhesion, proliferation, migration, and angiogenesis. RADA16-I provided a three-dimensional environment for DPSCs. Besides, the SCF promoted HUVECs to form more vascular-like structures and release more vascular endothelial growth factor A. In summary, the SCF-loaded RADA16-I scaffold improved adhesion, proliferation, and migration of DPSCs and the formation of more vascular-like structures of HUVECs. SCF-RADA16-I holds promise for guided pulp regeneration, and it can potentially be applied widely in tissue engineering and translational medicine in the future.
Highlights
Dental pulp tissue, rich in nerves and blood vessels, is an important component of teeth, which plays an important role in forming dentin, nutrition, sensation, and defense
In order to screen the optimal concentration of the stem cell factor (SCF), the proliferation of dental pulp stem cells (DPSCs) was evaluated
The SCF was found to promote the migration of dental progenitor cells and neovascularization.[23]
Summary
Rich in nerves and blood vessels, is an important component of teeth, which plays an important role in forming dentin, nutrition, sensation, and defense. Root canal therapy is a common treatment option for dentistry. During this process, the inflamed or necrotic pulp is removed and filled with synthetic material. Root canal therapy has proven to be quite useful, the remaining tooth structure is inactive and brittleness increases due to the loss of the natural pulp.[1,2] Young permanent teeth have a certain ability to regenerate dental pulp, but mature dental pulp is difficult to regenerate. Many researchers have devoted themselves to dental pulp regeneration
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