Abstract
Compendia of large-scale datasets made available in public repositories provide a precious opportunity to discover new biomedical phenomena and to fill gaps in our current knowledge. In order to foster novel insights it is necessary to ensure that these data are made readily accessible to research investigators in an interpretable format. Here we make a curated, public, collection of transcriptome datasets relevant to human placenta biology available for further analysis and interpretation via an interactive data browsing interface. We identified and retrieved a total of 24 datasets encompassing 759 transcriptome profiles associated with the development of the human placenta and associated pathologies from the NCBI Gene Expression Omnibus (GEO) and present them in a custom web-based application designed for interactive query and visualization of integrated large-scale datasets( http://placentalendocrinology.gxbsidra.org/dm3/landing.gsp). We also performed quality control checks using relevant biological markers. Multiple sample groupings and rank lists were subsequently created to facilitate data query and interpretation. Via this interface, users can create web-links to customized graphical views which may be inserted into manuscripts for further dissemination, or e-mailed to collaborators for discussion. The tool also enables users to browse a single gene across different projects, providing a mechanism for developing new perspectives on the role of a molecule of interest across multiple biological states. The dataset collection we created here is available at: http://placentalendocrinology.gxbsidra.org/dm3.
Highlights
We aimed to make available via an interactive web-based application a collection of transcriptome datasets curated from the Gene Expression Omnibus (GEO) public repository for their relevance to human placental development and pathology
Trophoblasts are the first cells differentiating from the fertilized egg, and eventually fusing with each other: a process transforming the monolayer cytotrophoblasts into syncytiotrophoblasts[3]
Several morphological and biochemical changes occur during this fusion process throughout pregnancy[4] to guarantee the development and appropriate functionality of the placenta[1]
Summary
The second version of the manuscript includes additional validation results presented in an additional figure
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