Abstract
The culture unit described was designed to provide a method whereby the experimental condition of the substrate solution can be maintained or manipulated while retaining the axenic integrity of the system. The culture unit was equipped with a modified polypropylene lid allowing entry and exit of treatment solutions that had been previously sterilized. Loblolly pine (Pinus taeda L.) inoculated by Pisolithus tinctorius (Pers.) Coker and Couch was grown axenically for up to six months in this culture unit system. Growth of roots and mycorrhizal morphology in this system were similar to those observed in other systems.
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