Abstract
Citrate synthase (EC 4. 1. 3. 7.) from pig heart and chicken heart muscle can be crystallized from 10 mM phosphate buffer (pH 7. 0–7. 5) and 10–15% PEG4000 solution. The space group is P4 12 12 with one subunit/asymmetric unit for the pig heart enzyme. The chicken heart citrate synthase purified from Blue-dextran as well as ATP-Sepharose affinity chromatography both crystallize in space group P2 12 12 with one molecule/asymmetric unit, but they have different unit cell dimensions. The a-axis differs by about 17 Å between these two crystal forms, while b- and c-axis dimensions are virtually identical.
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