Abstract
Since Gomori (1939) and Takamatsu (1939) developed practically identical methods for the demonstration of alkaline phosphatase in microscopic sections, a number of publications on modifications of the original technique have been issued. These modifications are claimed to give more reliable and accurate results than the original method. The difficulties encountered are chiefly concerned with the loss of enzyme activity in the process of the fixation and embedding of the tissue specimens. Danielli (1946) and Stafford & Atkinson (1948) have shown that fixation in chilled acetone or alcohol and paraffin embedding inactivate the original enzyme by more than 70 per cent.
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