Abstract
A New, sensitive spectrophotometric method for determination of Naproxen (Nap) has been proposed. The method is based on a modification of Naproxen into a hydroxy analog (mNap). The modified compound is coupled with the diazotized p-aminobenzoic acid (PABA) in alkaline medium, the resulting orange azo dye exhibit a maximum absorption at 500 nm. Beer's law is obeyed over the concentration range from 10 to 650 µg/20 ml, (i.e. 0.5-32.5 µg/ ml) with a good sensitivity (molar absorptivity 2.14x104 l.mol-1.cm-1), good precision (RSD% better than ±1.93%) and high accuracy (relative error % better than 0.43%), Sandell's sensitivity index is 0.0107µg.cm-2 and the limit of detection (LOD) is 0.024 µg/ml and the limit of quantitation (LOQ) is 0.082 µg/ml. The method has been applied successfully for determination of Naproxen in dosage forms after extraction of the active ingredient. .
Highlights
A New, sensitive spectrophotometric method for determination of Naproxen (Nap) has been proposed
Naproxen (Nap) is an analgesic, anti-inflammatory drug with antipyretic activities largely related to inhibition of prostaglandin synthesis (Karch, 2010), this drug is used more and more frequently because it blocks RNA-binding groove of nucleoprotein of virus and may have antiviral activity against influenza (Lejal et al.,2013)
A few methods for quantitative determination of Naproxen have been reported, these including HPLC (Wahbi et al.,2009) (Tashtoush and Al-Taani, 2003) (Muneer et al, 2017), RPHPLC (Pansara et al, 2013), LC-MS (Veeragoni et al, 2016), electrochemical method (Soltani et al, 2018), Ion selective electrode (Lenik et al, 2002), chemiluminescence (Yinhuan and Jiuru, 2006), Conductometric (Alhazmiand and Al Bratty, 2017), While other papers report a spectrophotometric assay of Naproxen at uv region (Nijhu et al.,2011) (Maheshwari et al, 2010) (Sloka et al.,2011) (Jain et al.,2011)
Summary
A few methods for quantitative determination of Naproxen have been reported, these including HPLC (Wahbi et al.,2009) (Tashtoush and Al-Taani, 2003) (Muneer et al, 2017), RPHPLC (Pansara et al, 2013), LC-MS (Veeragoni et al, 2016), electrochemical method (Soltani et al, 2018), Ion selective electrode (Lenik et al, 2002), chemiluminescence (Yinhuan and Jiuru, 2006), Conductometric (Alhazmiand and Al Bratty, 2017), While other papers report a spectrophotometric assay of Naproxen at uv region (Nijhu et al.,2011) (Maheshwari et al, 2010) (Sloka et al.,2011) (Jain et al.,2011). A colorimetric assay of naproxen tablets based on chemical derivatization with the chromogenic reagent 4-carboxyl-2,6-dinitrobenzene diazonium ion was developed. The optimal reaction time was found to be 5 min at 30 oC after vortex mixing of the drug/reagent mixture for 10 s. Naproxen was determined by formation of ion-pair complex with bromophenol blue,the colored product was measured at 432 nm (Keyhanian et al.,2014), another ion-pair procedure based on reaction between Naproxen with two sulfophthalein acid dyes, namely bromocresol green and bromothymol blue (Alizadeh and Keyhanian, 2015). The aim of this work is determination of naproxen by indirect spectrophotometric method after modification of Nap to hydroxyl analog, coupling with diazotized p-aminobenzoic acid (PABA)
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