A covalent organic framework for chiral capillary electrochromatography using a cyclodextrin mobile phase additive.

Abstract

Covalent organic frameworks (COFs) have been recognized as promising solid phases in capillary electrochromatography (CEC). Imine-based COF-coated open-tubular CEC column (COF TpBD-coated OT column) was prepared and characterized by X-ray diffraction (XRD), Fourier-transform infrared (FT-IR) spectra, thermogravimetric analysis (TGA), nitrogen adsorption/desorption (Brunauer-Emmett-Teller [BET]), and scanning electron microscopy (SEM). The results showed that the column efficiency was up to 26,776 plate/m, and the thickness of stationary phase was about 6.00 μm for the column prepared under the optimal conditions. Enantioseparation of 15 kinds of the single chiral compounds (histidine, arginine, lysine, leucine, threonine, methionine, valine, aspartic acid and glutamic acid, fipronil, diclofop, imazamox, quizalofop-p, imazethapyr, and acephate) and 3 kinds of mixed amino acids racemaces (valine, methionine, and glutamic acid) were performed with three methods: capillary electrochromatography with COF TpBD-coated OT column (Method 1), CEC with COF TpBD-coated OT column as the separation channels, and capillary electrophoresis (CE) with HP-β-CD as the chiral mobile phase additive (Method 2) and CE with HP-β-CD as the chiral mobile phase additive (Method 3). Separation efficiency and chiral selectivity of Method 2 was best among the three methods. Under the optimal separation conditions of Method 2, all the enantiomers reached the baseline separation regardless of the single chiral compounds or the mixed amino acids. Relative standard deviation (RSDs) of the mean column efficiency for reproducibility and stability was in the range of 0.46-1.49%. This combination of CEC and CE has great potential for use in chiral separation.