A cost effective strategy for dual channel optical sensing of adrenaline based on 'in situ' formation of copper nanoparticles.

Abstract

Assessment of adrenaline (ADR) levels in biological fluids and pharmaceutical formulations is of prime importance due to its association with many disease conditions. Here a novel, cost effective, dual channel sensing strategy is developed for ADR based on in situ formation of copper nanoparticles. The proposed sensor works via both fluorimetry and colorimetry. Visual detection was also enabled by color change of solution from pale blue to reddish brown. Here CuCl2 solution is used as probe to simplify method and was function as excellent fluorimetric as well as colorimetric ADR sensor. Fabricated sensor is very simple, selective and reproducible in nature. Proposed sensor works fluorimetrically in linear range of 3.00 × 10-5 to 5.00 × 10-7 M and colorimetrically in linear range of 5.00 × 10-4 to 2.00 × 10-5 M. Artificial urine and commercial pharmaceutical formulations were successfully analyzed as samples for estimation of ADR by developed dual channel sensor.