Abstract

Non-invasive determination of leaf nitrogen (N) and water contents is essential for ensuring the healthy growth of the plants. However, most of the existing methods to measure them are expensive. In this paper, a low-cost, portable multispectral sensor system is proposed to determine N and water contents in the leaves, non-invasively. Four different species of plants—canola, corn, soybean, and wheat—are used as test plants to investigate the utility of the proposed device. The sensor system comprises two multispectral sensors, visible (VIS) and near-infrared (NIR), detecting reflectance at 12 wavelengths (six from each sensor). Two separate experiments were performed in a controlled greenhouse environment, including N and water experiments. Spectral data were collected from 307 leaves (121 for N and 186 for water experiment), and the rational quadratic Gaussian process regression (GPR) algorithm was applied to correlate the reflectance data with actual N and water content. By performing five-fold cross-validation, the N estimation showed a coefficient of determination () of 63.91% for canola, 80.05% for corn, 82.29% for soybean, and 63.21% for wheat. For water content estimation, canola showed an of 18.02%, corn showed an of 68.41%, soybean showed an of 46.38%, and wheat showed an of 64.58%. The result reveals that the proposed low-cost sensor with an appropriate regression model can be used to determine N content. However, further investigation is needed to improve the water estimation results using the proposed device.

Highlights

  • IntroductionLeaf nitrogen (N) determination in crop plants is crucial since it plays an essential role in plant growth and development, including photosynthesis and important enzyme production [1,2]

  • Leaf nitrogen (N) determination in crop plants is crucial since it plays an essential role in plant growth and development, including photosynthesis and important enzyme production [1,2].Application of N fertilizer at an optimum level for different growth stages is challenging, as the amount of N in soil is limited [3]

  • Canola showed an R2 of 18.02%, corn showed an R2 of 68.41%, soybean showed an R2 of 46.38%, and wheat showed an R2 of 64.58%

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Summary

Introduction

Leaf nitrogen (N) determination in crop plants is crucial since it plays an essential role in plant growth and development, including photosynthesis and important enzyme production [1,2]. Application of N fertilizer at an optimum level for different growth stages is challenging, as the amount of N in soil is limited [3]. Excessive N application affects the environment in several ways such as denitrification of soil, global warming, and water pollution [6]. The current techniques include destructive and non-destructive approaches. Invasive determinations are basically chemical methods [7], namely, Kjeldahl digestion and Dumas combustion. There exist two approaches to determine plant N non-invasively, including light spectroscopy and hyperspectral imaging.

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