A cosmid vector containing a dominant selectable marker for cloning Chlamydomonas genes by complementation

Abstract

Cosmid vectors containing a dominate selectable marker ( ble) for complementation cloning of genes in Chlamydomonas reinhardtii were created. The usefulness of these vectors, which differ in the orientation of the ble cassette, was demonstrated by transforming C. reinhardtii to phleomycin resistance, by constructing a large library (∼5×10 5 recombinants) in one of them using DNA from a C. reinhardtii mutant, and by transforming C. reinhardtii with recombinant cosmid clones and pools.