Abstract
Objective: The purpose of this study was to evaluate the correlation between CT perfusion parameters and the hypoxia-inducible factor-1 alpha (HIF-1α), vascular en-dothelial growth factor (VEGF), matrix metalloproteinase-2 (MMP-2) and microvessel density (MVD) marked by CD34 molecular of rabbit VX2 liver tumors and to investigate the value of CT perfusion imaging in evaluating tumor angiogenesis. Material and methods: Twenty-four cases of rabbit VX2 liver tumor were performed by CT perfusion scanning. Hepatic artery perfusion (HAP), portal vein perfusion (PVP), total hepatic blood flow (THBF) and hepatic perfusion index (HPI) were measured by perfusion software. HIF-1α, VEGF and MMP-2 expression and MVD were detected in the 24 rabbit VX2 liver tumor tissue samples using immunohistochemical method. The correlation between the HIF-1α, VEGF, MMP-2 expression and MVD and CT perfusion parameters were analyzed. Results: Correlation analysis revealed that the expression of HIF-1α, MMP-2, MVD were positively related to the HAP, THBF, HPI (p 0.05); and correlation analysis revealed that the expression of VEGF was positively related to the HAP, HPI (p 0.05). There was a positive relationship between the expression of HIF-1α, VEGF, MMP-2 and MVD (p < 0.01). Conclusions: CT perfusion imaging can reflect the blood perfusion of the rabbit VX2 liver tumors and evaluate the information of angiogenesis about tumors.
Highlights
The purpose of this study was to evaluate the correlation between Computed tomography (CT) perfusion parameters and the hypoxia-inducible factor-1 alpha (HIF-1α), vascular endothelial growth factor (VEGF), matrix metalloproteinase-2 (MMP-2) and microvessel density (MVD) marked by CD34 molecular of rabbit VX2 liver tumors and to investigate the value of CT perfusion imaging in evaluating tumor angiogenesis
Correlation analysis revealed that the expression of HIF-1α, MMP-2, MVD were positively related to the Hepatic artery perfusion (HAP), total hepatic blood flow (THBF), hepatic perfusion index (HPI) (p < 0.01), but no relations with portal vein perfusion (PVP) (p > 0.05); and correlation analysis revealed that the expression of VEGF was positively related to the HAP, HPI (p < 0.01), but no relations with PVP and THBF (p > 0.05)
Microvessel density (MVD) measurement is the gold standard for quantitative analysis of tumor angiogenesis, and MVD is an important indicator that reflects the biological behavior of malignant tumors [8] [9]
Summary
Tumor angiogenesis is regulated by various angiogenic factors. Hypoxia-inducible factor-1 alpha (HIF-1α) is the key regulator of angiogenesis in hypoxia, which directly and indirectly affects angiogenesis by influencing the expression of other angiogenic growth factors, and plays a vital role in the evolution of malignant tumors [3]-[5]. Matrix metalloproteinase-2 (MMP-2) enhances tumor cell invasion and metastasis into surrounding tissue by promoting tumor angiogenesis and extracellular matrix degradation [7]. The study of angiogenesis in tumor tissue is extremely valuable for determining the biological characteristics of tumors and evaluating treatment efficacy, prognosis, and other factors. Microvessel density (MVD) measurement is the gold standard for quantitative analysis of tumor angiogenesis, and MVD is an important indicator that reflects the biological behavior of malignant tumors [8] [9]. Computed tomography (CT) perfusion imaging is a new functional imaging technique that can quantitatively measure blood perfusion in tissues and organs and thereby reflect these tissues’ and organs’ microcirculation characteristics [11]-[17]
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