Abstract
The pH fluorescence indicator molecules could serve as sensitive sensors for an optical pH measuring technique in various body fluids and tissues (Tageeva et al., 1971; Lübbers and Opitz, 1976; Opitz, 1976; Lübbers et al., 1977; Peterson et al., 1980), if one could eliminate ionic strength interference which causes spectral changes indistinguishable from those caused by pH. To obtain the true optically measured pH value, a correction method for ionic strength changes has been developed based on a double pH indicator system, i.e., hydroxypyrenetrisulfonic acid (HPTS, introduced by Wolfbeis et al., 1983) and ß-methylumbelliferone (ß-M), (Bülow and Dieck, 1928; Chen, 1968; Nakashima et al., 1972).
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