Abstract
: Artificial intelligence (AI) trained with a convolutional neural network (CNN) is a recent technological advancement. Previously, several attempts have been made to train AI using medical images for clinical applications. However, whether AI can distinguish microscopic images of mammalian cells has remained debatable. This study assesses the accuracy of image recognition techniques using the CNN to identify microscopic images. We also attempted to distinguish between mouse and human cells and their radioresistant clones. We used phase-contrast microscopic images of radioresistant clones from two cell lines, mouse squamous cell carcinoma NR-S1, and human cervical carcinoma ME-180. We obtained 10,000 images of each of the parental NR-S1 and ME-180 controls as well as radioresistant clones. We trained the CNN called VGG16 using these images and obtained an accuracy of 96%. Features extracted by the trained CNN were plotted using t-distributed stochastic neighbor embedding, and images of each cell line were well clustered. Overall, these findings suggest the utility of image recognition using AI for predicting minute differences among phase-contrast microscopic images of cancer cells and their radioresistant clones. SIGNIFICANCE: This study demonstrates rapid and accurate identification of radioresistant tumor cells in culture using artifical intelligence; this should have applications in future preclinical cancer research.
Highlights
There has been a remarkable development in image recognition technology based on artificial intelligence (AI) trained with a machine learning method, called deep learning
Features extracted by the trained convolutional neural network (CNN) were plotted using t-distributed stochastic neighbor embedding, and images of each cell line were well clustered. These findings suggest the utility of image recognition using AI for predicting minute differences among phase-contrast microscopic images of cancer cells and their radioresistant clones
One of the leading neural networks used in image recognition is the convolutional neural network
Summary
There has been a remarkable development in image recognition technology based on artificial intelligence (AI) trained with a machine learning method, called deep learning. Popularized image recognition using deep learning [7]. In deep learning, multilayered learning circuits called neural networks simulate human neurons. One of the leading neural networks used in image recognition is the convolutional neural network The CNN is organized on the basis of the human visual system and is a robust network against image shift [9]. Deep learning warrants substantial training data to enhance the performance of the CNN, especially with deep multilayered networks. In transfer learning, learning efficiency is enhanced by optimizing the parameters of only the higher layers without altering the lower layers
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