A convergent synthetic platform of photocurable silk fibroin-polyvinylpyrrolidone hydrogels for local anaesthesia examination

Abstract

Investigations may quickly and easily convert a sol-gel into a gel-like substance-using photocurable hydrogel, which has several potential uses in biomedical research. N-Vinyl-2-pyrrolidone (NVP) monomer has been converted into Polyvinylpyrrole (PVP) and interacting matrix with silk fibroin macromolecule hydrogel by photoinitiated polymerization in this study. MTT experiment and microscopic inspections proved the PVP/SF hydrogel's biocompatibility with L929 fibroblast cell lines (96% cell survival and growth compatibility). This hydrogel's release pattern is unique from other hydrogels because of its delayed and low cumulative release. Mice models were used to show treated materials' anaesthetic and toxicological effects. Additionally, PVP/SF@LDC hydrogels dramatically suppress in vivo tumour development in mouse models and efficiently minimize their in vivo toxicity. In vitro and in vivo testing revealed that encapsulating LDC in PVP/SF hydrogels improved the drug's anaesthetic activity while extending its duration. Experimental research involving the controlled release of local anaesthetics might make excellent use of this new approach.