A convenient renewable surface plasmon resonance chip for relative quantification of genetically modified soybean in food and feed.

Alexandra Plácido,Cristina Delerue-Matos,José Roberto S A Leite,Noemí De-Los-Santos-Álvarez,Frederico Ferreira-Da-Silva,Guangyuan He

doi:10.1371/journal.pone.0229659

Abstract

The cultivation of genetically modified organisms (GMO) continues to expand worldwide. Still, many consumers express concerns about the use of GMO in food or feed, and many countries have legislated on labelling systems to indicate the presence of GMO in commercial products. To deal with the increased number of GMO events and to address related regulations, alternative detection methods for GMO inspection are required. In this work, a genosensor based on Surface Plasmon Resonance under continuous flow was developed for the detection and quantification of a genetically modified soybean (event GTS 40-3-2). In a single chip, the simultaneous detection of the event-specific and the taxon-specific samples were achieved, whose detection limits were 20 pM and 16 pM, respectively. The reproducibility was 1.4%, which supports the use of the chip as a reliable and cost-effective alternative to other DNA-based techniques. The results indicate that the proposed method is a versatile tool for GMO quantification in food and feed samples.

Highlights

Modified organisms (GMOs) are plants, animals, or microorganisms whose genetic composition had been altered by genetic engineering methods through the insertion of a new gene or by deletion of an existing one, in order to express a desired characteristic [1]
With the objective of developing a sensitive platform for genetically modified organisms (GMO) quantification, a novel SPRbased biosensor was designed targeting the transgenic construct of Roundup Ready (RR) soybean and the taxon-specific soybean gene, lectin
We report the first Surface plasmon resonance (SPR) biosensor developed for GMO quantification in feed and food samples according to the legislation guidelines that do not rely on qPCR or labels for detection

Summary

Introduction

Modified organisms (GMOs) are plants, animals, or microorganisms whose genetic composition had been altered by genetic engineering methods through the insertion of a new gene or by deletion of an existing one, in order to express a desired characteristic [1]. These modifications have contributed to the sustainable increase in productivity and to enhancing the chemical profile and nutritional quality of the derived products [2]. The soybean GTS 40-3-2 event was developed to allow the use of glyphosate, an herbicide, as a weed control option [4]. This GM soybean variety contains the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS)

Methods

Results

Conclusion