Abstract
A method for measuring clearing factor in vitro has been developed. This method makes use of a standard substrate–receptor system obtained by adding constant amounts of a commercial fat emulsion to stored plasma. Best results are obtained when plasma which has been heated to 56 °C for 5 minutes and stored at −20 °C is used at 50% concentration. A comparison of the production of clearing factor in different species using plasma from different species was made. With all clearing factors, the human and dog plasmas are superior to the other species studied. The results of intravenous and depot injections of heparin are compared.The intravenous injections cause maximum prolongation of clotting time almost immediately and maximum amounts of clearing factor within 30 minutes. Depot injections stimulate the production of maximum amounts of clearing factor as quickly as intravenous injections, but the maximum effect on the clotting mechanism is delayed for three to four hours.The implications of these results are discussed. The application of the method in the study of diseases associated with abnormalities in plasma proteins and fat metabolism is suggested.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
