Abstract
The proton release associated with the iso-pH p/iso-P CO 2 oxygenation of human whole blood ( ΔH + ΔO 2 ratio) was measured on blood samples made mildly alkalotic by isotonic addition of base. The results, together with those obtained previously by the same technique on blood of lower buffer base content, suggest that the −ΔH + ΔO 2 ratio of whole blood is a complex function of both buffer base content and P CO 2 ; in particular, a trend corresponding to the steep pH-dependency of the Bohr effect described by Rossi and Roughton in Hb solutions was apparent over the limited pH range covered by both sets of data
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