Abstract

A preparative method of starch gel electrophoresis is described in which the electrophoretically separated components of a mixture are eluted directly and continuously from the starch gel. The method is simple and convenient and permits efficient separations with high recovery of materials. Three examples of its use in the separation of protein mixtures are provided: a calf thymus histone fraction, a mixture of lysozyme with β-lactoglobulin, and normal human serum. A tray of variable depth, for use in analytical starch gel electrophoresis experiments, is also described.

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