Abstract
Quantitative aspects of C1 metabolism by chemostat cultures of Pichia pastoris have been studied. Under methanol limitation the maximum growth yield was 0.41 (g/g), the maintenance energy was 9.1 mg CH3OH/g.h and the μmax was estimated to be 0.18 h−1. Formate was utilized by methanol-limited cultures, and increased the cell yield approx. 20% as compared to that on methanol alone.
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