Abstract
A multi-stage, continuous culture apparatus has been designed and tested for the production of algae for larval molluscs and crustacea. A single-line system produced a maximum of 2.4 × 10 11 cells/day, or 5 g ash-free dry weight of Monochrysis lutheri. Multiple-line systems are recommended for hatcheries. The flow rate affected algal cell density, yield, biomass, protein level, and residual nitrate. Maximal cell yield occurred at 10 I flow per day, a dilution rate of 63% of the volume of the first growth carboy, or 30% of the volume of the total system. The system is also adaptable to growth of larger planktonic algae or mixed cultures of algae and protozoa and/or rotifers.
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