Abstract
Bay scallop (Argopecten irradians) is one of the most economically important aquaculture species in China. In this study, we constructed a consensus microsatellite-based genetic linkage map with a mapping panel containing two hybrid backcross-like families involving two subspecies of bay scallop, A. i. irradians and A. i. concentricus. One hundred sixty-one microsatellite and one phenotypic (shell color) markers were mapped to 16 linkage groups (LGs), which corresponds to the haploid chromosome number of bay scallop. The sex-specific map was 779.2 cM and 781.6 cM long in female and male, respectively, whereas the sex-averaged map spanned 849.3 cM. The average resolution of integrated map was 5.9 cM/locus and the estimated coverage was 81.3%. The proportion of distorted markers occurred more in the hybrid parents, suggesting that the segregation distortion was possibly resulted from heterospecific interaction between genomes of two subspecies of bay scallop. The overall female-to-male recombination rate was 1.13∶1 across all linked markers in common to both parents, and considerable differences in recombination also existed among different parents in both families. Four size-related traits, including shell length (SL), shell height (SH), shell width (SW) and total weight (TW) were measured for quantitative trait loci (QTL) analysis. Three significant and six suggestive QTL were detected on five LGs. Among the three significant QTL, two (qSW-10 and qTW-10, controlling SW and TW, respectively) were mapped on the same region near marker AiAD121 on LG10 and explained 20.5% and 27.7% of the phenotypic variance, while the third (qSH-7, controlling SH) was located on LG7 and accounted for 15.8% of the phenotypic variance. Six suggestive QTL were detected on four different LGs. The linkage map and size-related QTL obtained in this study may facilitate marker-assisted selection (MAS) in bay scallop.
Highlights
Linkage maps have become important genetic tools which provide detailed information on genotype-phenotype relationships [1]
The number of segregating markers was essentially similar between the two families, with 137 in family CC5 and 119 in family CC10
A consensus genetic linkage map was constructed for the bay scallop using microsatellite markers, providing a baseline map for this important aquaculture species
Summary
Linkage maps have become important genetic tools which provide detailed information on genotype-phenotype relationships [1]. Marine bivalves represent a major portion in the aquaculture industry, only a few linkage maps have been constructed mainly using amplified fragment length polymorphisms (AFLPs). These AFLP-based linkage maps include the oysters (Crassostrea virginica [8], Crassostrea gigas [9] and Ostrea edulis [10]), the pearl oysters (Pinctada martensii [11] and Pinctada fucata [12]), the mussel Mytilus edulis [13] and the scallops (Chlamys farreri [14,15] and Argopecten irradians [16,17]). A few QTL controlling growth and disease resistance traits have been located on some linkage maps in marine bivalve such as the oysters [19,20,21] and the scallops [16,18], which will be useful for MAS in these aquaculture species
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