A concentric 3D-printed centrifuge rotor to study the effects of hypergravity on small organisms at three different but concurrent radial accelerations

Abstract

For life to reach space, it must first escape Earth's gravity—hence exposure to hypergravity during launch, and interplanetary acceleration and deceleration. Long-term colonisation of space requires the establishment of sustainable, in situ, food production systems. Earthworms may be involved as a regolith/soil ameliorant, a source of protein, and for organic waste conversion. Earthworms, however, have hydrostatic skeletons that may be susceptible to hypergravity exposure. We investigated whether earthworms (Eisenia andrei) would survive prolonged centrifugal hypergravity using a table-top centrifuge. Our intention was to concurrently test three different hypergravity treatments (plus a 1 G control). Our concentric rotor concept, with appropriate design adaptations, allows concurrent testing of three different gravity treatments on small- and microorganisms, cell cultures, plants, microcosm, and chemical/biochemical reactions for extended periods of hours to months. Our concentric concept therefore is easily adaptable and may facilitate other terrestrial-based research on chemical, molecular, and organismal levels.•We designed a 3D-printed centrifuge rotor with three concentric rings, each ring with four separate 125 mL compartments.•This design was tested with an 8-day pilot run with four young worms per container at 1.7 G, 2.5 G, and 3.1 G, with a 1 G control.•All worms at all treatments gained body mass, the mean increases of which were almost indistinguishable between treatments, illustrating the utility of the design.