Abstract
Avian mycoplasma is a bacterial disease causing chronic respiratory disease (CRD) in poultry industries with high economic losses. The eradication of this disease still remains as a challenge. A multi-epitope prophylactic vaccine aiming the antigenic proteins of Mycoplasma gallisepticum can be a capable candidate to eradicate this infection. The present study is focused to design a multi-epitope vaccine candidate consisting of cytotoxic T-cell (CTL), helper T-cell (HTL), and B-cell epitopes of antigenic proteins, using immunoinformatics strategies. The multi-epitopic vaccine was designed, and its tertiary model was predcited, which was further refined and validated by computational tools. After initial validation, molecular docking was performed between multi-epitope vaccine construct and chicken TLR-2 and 5 receptors, which predicted effective binding. The in silico results specify the structural stability, precise specificity, and immunogenic response of the designed multi-epitope vaccine, and it could be an appropriate vaccine candidate for the M. gallisepticum infection.
Highlights
Mycoplasma gallisepticum is cell-wall-less bacteria, the key avian respiratory pathogen which is the causative agent for chronic respiratory disease (CRD) in chickens and infectious sinusitis in turkeys and house finches [1]
M. gallisepticum infection tends to intensify the pathology of accompanying viral infections like Newcastle disease virus (NDV), infectious bronchitis (IB), and CRD in chickens
The epitopes from the antigenic proteins of M. gallisepticum with length ranging from 10 to 15 peptides are given in Supplementary Table S3
Summary
Mycoplasma gallisepticum is cell-wall-less bacteria, the key avian respiratory pathogen which is the causative agent for chronic respiratory disease (CRD) in chickens and infectious sinusitis in turkeys and house finches [1]. M. gallisepticum infection tends to intensify the pathology of accompanying viral infections like Newcastle disease virus (NDV), infectious bronchitis (IB), and CRD in chickens. M. gallisepticum is the crucial pathogen in poultry industries leading to significant economic loss by infecting layers and breeder and broiler poultry flocks [2]. M. gallisepticum has a small genome with 996 kb responsible for its limited biosynthetic capabilities and low replication rates and tends to infect the respiratory tract and colonize in the mucosal surface tract of chicken. Transmission of M. gallisepticum in respiratory tract mucosa induces local
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