Abstract
C.botulinum ATCC 3502 is an obligate rod-shaped spore forming anaerobe causing food poisoning cases worldwide. The increased burden of sporadic hospital outbreaks reflects the pandemicity caused by this pathogen. Several attempts to control the disease surveillance, emergence of antibiotic resistant strains plagued the defense provided by the drugs. Peptidoglycan biosynthesis of C.botulinum ATCC 3502 is targeted to identify potential drug targets due to presence of no functional homolog in host (Homo sapiens). The role of peptidoglycan is to provide strength, fragility and protection to bacterial cells; therefore, it is considered as an attractive target for drug target identification. This study involves systematic exploration of targeted pathway by performing metabolic pathways analysis in two phases (1) mathematical modeling (2) elementary mode analysis (EM). Performing stoichiometric and kinetic modeling discerns the steady state conditions of the system to scrutinize elementary nodes with well-defined objective function. The study identifies Mur ligase enzymes (murA, murB, murC, murD), D-alanine-D-alanine ligase and glutamate racemase potential therapeutic targets for drug discovery. Further, the quantitative analysis characterized their potential in causing the pathogenicity. Physicochemical characterization and subcellular localization analysis assist in understanding biological activity of identified elementary nodes under different environmental conditions.
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