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Abstract
Honeybees are pivotal pollinators of crops and wild flora, and of great importance in supporting critical ecosystem balance. Nosema ceranae, a unicellular fungal parasite that infects midgut epithelial cells of honeybees, can dramatically reduce honeybee population and productivity. Here, midguts of Apis mellifera ligustica workers at 7 d and 10 d post inoculation (dpi) with sucrose solution (Ac7CK and Ac10CK) and midguts at 7 dpi and 10 dpi with sucrose solution containing N. ceranae spores (Ac7T and Ac10T) were sequenced using strand-specific cDNA library construction and next-generation sequencing. A total of 1956129858 raw reads were gained in this article, and after quality control, 1946489304 high-quality clean reads with a mean Q30 of 93.82% were obtained. The rRNA-removed clean reads were then aligned to the reference genome of Apis mellifera with TopHat2. For more insight please see “Genome-wide identification of long non-coding RNAs and their regulatory networks involved in Apis mellifera ligustica response to Nosema ceranae infection” [1]. Raw data were deposited in NCBI Sequence Read Archive (SRA) database under the BioProject number PRJNA406998. These data can be used for comparative analysis to identify differentially expressed coding RNAs and non-coding RNAs involved in A. m. ligustica responses to N. ceranae stress, and for investigation of molecular mechanisms regulating host N. ceranae -response.
Highlights
Honeybees are pivotal pollinators of crops and wild flora, and of great importance in supporting critical ecosystem balance
For more insight please see “Genome-wide identification of long non-coding RNAs and their regulatory networks involved in Apis mellifera ligustica response to Nosema ceranae infection” [1]
These data can be used for comparative analysis to identify differentially expressed coding RNAs and noncoding RNAs involved in A. m. ligustica responses to N. ceranae
Summary
Frames of sealed brood obtained from a healthy colony of A. m. ligustica located in the teaching apiary of College of Bee Science, Fujian Agriculture and Forestry University were kept in an incubator at 34 ± 0.5 C, 50% RH to provide newly emerged Nosema-free honeybees. ML of 50% sucrose solution containing 1 Â 106 spores of N. ceranae, as shown in Fig. 1B [1]. Control bees were inoculated in an identical manner using a 50% sucrose solution (w/w in water) without N. ceranae spores. Three replicate cages of 20 honeybees each were used in N. ceranae-treated and control groups. N. ceranae-treated and control workers' midguts were respectively harvested at 7 d or 10 d post inoculation (dpi), immediately frozen in liquid nitrogen and kept at À80 C until deep sequencing. Total RNA of the six midgut samples from N. ceranae-treated groups and six midgut samples from control groups were respectively extracted using Trizol (Life Technologies) according to the manufacturer's instructions, and examined via 1% agarose gel eletrophoresis. All RNA sequencing data produced in our study are available in NCBI SRA database and connected to BioProject PRJNA406998
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