Abstract
BackgroundColorectal cancer (CRC) is the 3rd most common cancer type in the world. The correlation between immune repertoire and prognosis of CRC has been well studied in the last decades. The diversity and stability of the immune cells can be measured by hypervariable complementarity‐determining region 3 (CDR3) segments of the T‐cell receptor (TCR).MethodsIn this study, we collected five healthy controls and 19 CRC patients’ peripheral blood mononuclear cells (PBMCs) in three stages, namely 1 day preoperative, 3 days’ postoperative, and 7 days’ postoperative, respectively. Simultaneously, we have also done the comparative analysis of these two different anesthesia methods, namely TIVA and CEGA. Sequencing of the TCR segments has been performed by multiplex PCR and high‐throughput next‐generation sequencing. We also analyzed the distribution of CDR3 length, highly expansion clones (HECs), TRBV, and TRBJ gene usage.ResultsOur result showed a significant difference between TCR CDR3 length distribution and HEC distribution between CRC patients and healthy controls. We also found that TRBV11‐2, TRBV12‐1, TRBV16, TRBV3‐2, TRBV4‐2, TRBV4‐3, TRBV5‐4, TRBV6‐8, TRBV7‐8, TRBV7‐9 and RBV11‐2, TRBV12‐1, TRBV16, TRBV3‐2, TRBV4‐2, TRBV4‐3, TRBV5‐4, TRBV6‐8, TRBV7‐8, and TRBV7‐9 usages are different between CRC patients and healthy controls.ConclusionIn conclusion, CRC patients were presented with different immune repertoire in comparison with healthy controls. In this study, significant difference in TRBV and TRBJ gene usage in between case and control group could provide some potential biomarker for the diagnosis and the treatment of the patients with CRC.
Highlights
Colorectal cancer (CRC) is the third most common cancer in the world and the fifth most prevalent cancer type in China, causing 376.3 thousand new patients and 191.0 mortality a year (Chen, Xu, et al, 2016a; Chen, Zheng, et al, 2016b; Wang et al, 2017)
We recruited 19 CRC patients and five healthy controls to study the difference in immune repertoire status among colorectal cancer patient at preoperative, postoperative, and healthy controls
In comparison with complementarity‐determining region 3 (CDR3) length distribution between preoperative colorectal patients and healthy controls showed there was significant difference between these two groups. This result again elucidated the immune repertoire effect on colorectal cancer patients which corresponds to the similar finding of previously reported CRC immune correlation study 1 (Li et al, 2016; Nakanishi et al, 2016)
Summary
Colorectal cancer (CRC) is the third most common cancer in the world and the fifth most prevalent cancer type in China, causing 376.3 thousand new patients and 191.0 mortality a year (Chen, Xu, et al, 2016a; Chen, Zheng, et al, 2016b; Wang et al, 2017). Further study on CRC patients in respect to change in immunological microenvironment with origin as well as the prognosis of cancer is a one of the significant methods for early detection of biomarkers as well as identifying the target for immunotherapy. We applied high‐throughput ‐generation sequencing (NGS) to elucidate the immune repertoire status among sporadic colorectal cancer patients (T1M0N0; Stage I) in different time points (1 day before surgery, 3 days’ after surgery, and 7 days after surgery) with different anesthesia methods to patients and healthy controls. Highly expanded clone distribution in preoperative patients, healthy controls, and postoperative patients at different time points with different anesthesia (TIVA and CGEA) methods has been compared in this study. In order to understand the mechanism of CRC immune exchange, TRBV, TRBJ gene repertoires between preoperative patients and healthy controls would be studied
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