A comprehensive root cause analysis of anammox bioreactor performance decline

Abstract

The cultivation of anaerobic ammonia oxidizing bacteria (anammox) has gained enormous awareness over the last few decades. Although numerous studies focus massively on successfully growing these anammox to different enrichment environments, in reality, the failure rates are somewhat comparable to the reported success rates. This study combines a variety of measurement techniques to observe and monitor the sequence of a bioreactor performance decline following elevated influent substrate concentration. After attaining stable substrate removal throughout a nitrogen loading rate (NLR) range of 0.691 to 1.669 kg-N·m−3·d−1, the performance of the lab-scale anammox-sequencing batch reactor (SBR) abruptly broke down as the NLR reached 2.01 kg-N·m−3·d−1. The gathered information showed that the increased NLR firstly caused a significant and unfavorable change in the free ammonia (FA) and free nitrous acid (FNA) concentration in the bioreactor. A subsequent drop in N2 production and a decline from a peak high of 0.381 to a low of 0.012 kg-N·kg-VSS−3·d−1 of the specific nitrogen removal rate (SNRR) led to an 82% absurd decline in microbial cellular energy production. Prior to these anammox switching to survival mode and secreting larger quantities (32% higher) of extracellular polymeric substances (EPS), the activity of syntrophic decomposers increased substantially leading to the internal production of excess CO2 in the bioreactor and thereby diverging the bioreactor pH to lower levels. The purposes of this study are to understand the reason an anammox process shows different signals during a decline phase and to enable immediate response to performance deterioration.