Abstract
Lipases that catalyze the hydrolysis of triglycerides play an outstanding role in developing biosensors used in various industries. With the latest developments and progress in biotechnology, nanobiotechnology and electronics, the demand for enzyme-based biosensors increases day by day. This review introduces different techniques and methods adopted to develop biosensors from lipase and discusses their properties and characteristics to demonstrate present scope, opportunities and challenges in biosensor technology. A focus of industries has shifted towards eco-friendly, high yield products with high profitability index. The use of enzymes seems to be the golden choice. With this review, we focus on the usage of an immobilized enzyme (lipase) in biosensors. We discuss various deficits in the immobilization and assay techniques, in brief, to bring about attention to the need for advancements in them to increase the overall accuracy of the biosensor in the present scenario.
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