Abstract
The soil-borne oomycete pathogen Aphanomyces euteiches causes devastating root rot diseases in legumes such as pea and alfalfa. The different pathotypes of A. euteiches have been shown to exhibit differential quantitative virulence, but the molecular basis of host adaptation has not yet been clarified. Here, we re-sequenced a pea field reference strain of A. euteiches ATCC201684 with PacBio long-reads and took advantage of the technology to generate the mitochondrial genome. We identified that the secretome of A. euteiches is characterized by a large portfolio of secreted proteases and carbohydrate-active enzymes (CAZymes). We performed Illumina sequencing of four strains of A. euteiches with contrasted specificity to pea or alfalfa and found in different geographical areas. Comparative analysis showed that the core secretome is largely represented by CAZymes and proteases. The specific secretome is mainly composed of a large set of small, secreted proteins (SSP) without any predicted functional domain, suggesting that the legume preference of the pathogen is probably associated with unknown functions. This study forms the basis for further investigations into the mechanisms of interaction of A. euteiches with legumes.
Highlights
Academic Editor: AnaThe Aphanomyces genus belongs to the order Saprolegniales, which includes filamentous eukaryotic pathogens that are encountered in different terrestrial and aquatic ecosystems [1,2]
We found that all the secretomes were closely related with respect to the functional classes of secreted proteins, but the ‘non-core’ secretomes were highly enriched in SSPs
In this paper, taking advantage of the availability of several A. euteiches strains adapted to pea or alfalfa, we investigated genetic variations that could be correlated with host preference, using a comparative genomics approach
Summary
The Aphanomyces genus belongs to the order Saprolegniales, which includes filamentous eukaryotic pathogens that are encountered in different terrestrial and aquatic ecosystems [1,2]. This genus includes about 45 species that infect plants, animals (fish, crustaceans) and agricultural crops [1,3,4,5,6]. It is of great interest to study Aphanomyces to reveal the evolutionary mechanisms that allow host adaptation. Aphanomyces euteiches is a soil-borne phytopathogenic oomycete with a wide spectrum of leguminous hosts and affects legumes such as pea, lentil and alfalfa [1,7,8]. A. euteiches induces root rot, which causes seedlings’ damping-off, yield decrease or even the death of the plant
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