Abstract
In recent years, Blautia has attracted attention for its role in ameliorating host diseases. In particular, Blautia producta DSM 2950 has been considered a potential probiotic due to its ability to mitigate inflammation in poly(I:C) induced HT-29 cells. Thus, to promote the development of indigenous intestinal microorganisms with potential probiotic function, we conducted a comprehensive experimental analysis of DSM 2950 to determine its safety. This comprised a study of its potential virulence genes, antibiotic resistance genes, genomic islands, antibiotic resistance, and hemolytic activity and a 14-day test of its acute oral toxicity in mice. The results indicated no toxin-related virulence genes in the DSM 2950 genome. Most of the genomic islands in DSM 2950 were related to metabolism, rather than virulence expression. DSM 2950 was sensitive to most of the tested antibiotics but was tolerant of treatment with kanamycin, neomycin, clindamycin, or ciprofloxacin, probably because it possessed the corresponding antibiotic resistance genes. Oral acute toxicity tests indicated that the consumption of DSM 2950 does not cause toxic side effects in mice. Overall, the safety profile of DSM 2950 confirmed that it could be a candidate probiotic for use in food and pharmaceutical preparations.
Highlights
Published: 23 April 2021It is estimated that there are 10–100 trillion microorganisms in the human gastrointestinal tract, including a variety of bacteria, viruses, archaea, and eukaryotes, with more than90% being members of the phyla Firmicutes or Bacteroidetes [1]
These microorganisms establish a symbiotic relationship with their host, and those that perform specific healthenhancing biological functions when ingested in adequate concentrations are known as probiotics [2]
Blautia positively contributes to biotransformation, relieves inflammatory and metabolic that Blautia positively contributes to biotransformation, relieves inflammatory and metadiseases, and has antimicrobial activity against certain microorganisms
Summary
90% being members of the phyla Firmicutes or Bacteroidetes [1] These microorganisms establish a symbiotic relationship with their host, and those that perform specific healthenhancing biological functions when ingested in adequate concentrations are known as probiotics [2]. Traditional probiotics, such as Lactobacillus spp. and Bifidobacterium spp., produce metabolites such as short-chain fatty acids, bacteriocins, and antimicrobial peptides, which can reduce inflammation, protect against pathogenic microbial infections, increase the integrity of the intestinal epithelium, and modulate immunity to promote host health [3,4,5,6]. Newly identified native intestinal bacteria such as Akkermansia, Bacteroides, Clostridium, and Blautia have rapidly attracted more attention for their health-promoting and therapeutic utility [8,9].
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