A Composite Mode Differential Gene Regulatory Architecture based on Temporal Expression Profiles.

Abstract

Exploring the complex interactive mechanism in a Gene Regulatory Network (GRN) developed using transcriptome data obtained from standard microarray and/or RNA-seq experiments helps us to understand the triggering factors in cancer research. The Transcription Factor (TF) genes generate protein complexes which affect the transcription of various target genes. However, considering the mode of regulation in a time frame such transcriptional activities are dependent on some specific activation time points only. It is also crucial to check whether the regulating capabilities are uniform across varied stages, especially when periodicity is a big issue. In this context, we propose an algorithm called RIFT which helps to monitor the temporal differential regulatory pattern of a Differentially Expressed (DE) target gene either by a TF gene or a group of TF genes from a large time series (TS) data. We have tested our algorithm on HeLa cell cycle data and compared the result with its most advanced state of the art counterpart proposed so far. As our algorithm yields up stringent mode and target specific significant valid TF genes for a DE gene, we can expect to have new forms of genetic interactions.