Abstract
The high-affinity IgE receptor, FcϵRI, is a key regulatory molecule in the allergic reaction. During the course of studies to find cis-acting elements for FcϵRI α chain gene expression, a CAGCTG sequence located in the first intron was revealed to serve as a crucial enhancer element. Electromobility shift assays using antibodies and in vitro translation products showed that the CAGCTG element was recognized by the USF1 / USF2 complex. As was the case for other intronic cis-elements, the CAGCTG element regulated the promoter in an orientation- and position-dependent manner. Overexpression of USF2 antisense repressed the FcϵRI α chain gene promoter and decreased the amount of α chain mRNA in mast cell lines. All these results indicated that the USF1 / USF2 complex activates the human FcϵRI α chain gene expression via the CAGCTG element in the first intron.
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