Abstract
An enzyme-linked immunosorbent assay which detects complement fixed in an antigen/antibody complex ( CFELISA) was used to examine sera previously examined by the complement fixation test ( CFT) in an investigation of enzootic abortion in ewes ( EAE). The CFELISA was easy to perform and did not suffer from the many drawbacks of the CFT. Accepting a CFT titre of ≥32 to indicate infection, the CFELISA had a sensitivity of 98 per cent and a specificity of 100 per cent; the predictive value of a positive result was 100 per cent and that of a negative result was 98 per cent.
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