Abstract
Nephropathy is characterized by urinary micro albumin. Rats are usually used in experimental studies. But, there was no specific and simple method for detecting rat urinary albumin. A specific, easily performed, and sensitive method for quantitatively determining rat urinary albumin is needed. Using rabbit anti‐rat albumin polyclonal antibody, biotinylated goat anti‐rabbit IgG, avidin conjugated peroxidase, and TMB (3,3′,5,5′‐tetramethylbenzidine) as substrate, a competitive ELISA for rat albumin in urine was developed. With this method, the urinary albumin in diabetic and normal rats was detected. This method was sensitive to 30 ng/mL and reproducibly quantifies rat urinary albumin levels in the range of 0.05–5 µg/mL. The rabbit anti‐rat albumin polyclonal antibody showed no cross reaction with bovine and human serum albumins and rat IgG, and showed little cross‐reaction with mouse albumin. The intra‐assay CV was less than 10%. The urinary albumin markedly increased in diabetic rats. Since quantifying urinary albumin was very important in the experimental study of diabetic nephropathy, the results from our experiments indicated that this competitive ELISA could be used for quantification of rat urinary albumin.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.