Abstract
Marine oxygen minimum zones (OMZs) are widespread regions of the ocean that are currently expanding due to global warming. While inhospitable to most metazoans, OMZs are hotspots for microbial mediated biogeochemical cycling of carbon, nitrogen and sulphur, contributing disproportionately to marine nitrogen loss and climate active trace gas production. Our current understanding of microbial community responses to OMZ expansion is limited by a lack of time-resolved data sets linking multi-omic sequence information (DNA, RNA, protein) to geochemical parameters and process rates. Here, we present six years of time-resolved multi-omic observations in Saanich Inlet, a seasonally anoxic fjord on the coast of Vancouver Island, British Columbia, Canada that undergoes recurring changes in water column oxygenation status. This compendium provides a unique multi-omic framework for studying microbial community responses to ocean deoxygenation along defined geochemical gradients in OMZ waters.
Highlights
Background & SummaryMarine oxygen minimum zones (OMZs), areas of low dissolved oxygen (O2) in subsurface waters, result from a combination of microbial respiration of organic matter raining down from surface waters and increased water column stratification[1,2,3]
As O2 becomes limiting, microbial communities shift their energy metabolism to use alternative terminal electron receptors in a thermodynamically defined order resulting in increased nitrogen loss and the production of climate active trace gases including nitrous oxide (N2O) and methane (CH4)[4,5,6,7,8,9]
Tag sequencing uses primers to amplify specific target genes and subsequently sequence them on high-throughput platforms, generating molecular barcodes that can be used to study microbial community structure and function depending on the marker
Summary
Background & SummaryMarine oxygen minimum zones (OMZs), areas of low dissolved oxygen (O2) in subsurface waters, result from a combination of microbial respiration of organic matter raining down from surface waters and increased water column stratification[1,2,3]. We present a compendium of multi-omic sequence information from the Saanich Inlet water column (Fig. 1) encompassing 412 SSU rRNA pyrotag (V6-V8 region) samples (Data Citation 1), 82 SSU 8. Samples for large volume (LV) SSU rRNA gene tags, metagenomics, metatranscriptomics, and metaproteomics were taken from six major depths spanning the oxycline (10, 100, 120, 135, 150, and 200 m).
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
