Abstract
Background: Cell block technique is a method of preparing cytological material so that it can be processed, sectioned, stained, and viewed as a histology section. The study compared two methods of cell block preparation, plasma-thromboplastin method and collodion bag technique of cell block preparation.
 Materials and Methods: Samples were randomly processed by plasma-thromboplastin technique or by collodion bag technique. Hematoxylin/Eosin stained cell block sections were examined and scored scaling from 1 to 3 for cellularity, clarity, nuclear features, cytoplasm, use of ancillary test and recovery of cell-cluster and fragments. Mann-Whitney U test was used for comparing two methods and establishing statistical significance.
 Results: The median cellularity score was 2.0 for plasma-thromboplastin and 1.0 for collodion bag. The median morphological preservation score and use of ancillary test score were 2.0 for both of the method while median score for recovery of cell cluster and fragments was 2.0 for collodion bag and 1.0 for plasma-thromboplastin. Statistically, both of the methods had similar results in cell block quality.
 The median total score for plasma-thromboplastin and collodion bag were 10.0 and 11.0 respectively. Diagnostic cell blocks were obtained in 46.51% of cell block by plasma-thromboplastin method and 60.52% of cell block by collodion bag method improving the diagnosis over conventional smear in 76.74% and 81.57% respectively.
 Conclusions: Cell block by plasma-thromboplastin and collodion bag method are cost effective, useful adjunct to FNA for diagnosis of mass lesion. Both techniques has no significant difference in quality of cell block produced.
Highlights
Fine-Needle aspiration cytology (FNAC) is a simple, inexpensive, atraumatic and safe cytodiagnostic technique for obtaining a tissue diagnosis of subcutaneous and other tumors
Aspirate obtained from FNAC was used for conventional smear preparation and the remaining portion was rinsed in 10% neutral buffered formalin and cell block was made either with PT technique or collodion bag (ColB) technique
Site-wise distribution and diagnosis made by cell block obtained by PT technique and ColB technique were shown in Table 2 and Table 3 respectively
Summary
Fine-Needle aspiration cytology (FNAC) is a simple, inexpensive, atraumatic and safe cytodiagnostic technique for obtaining a tissue diagnosis of subcutaneous and other tumors. It is valuable for preoperative assessment of lesions of the breast, thyroid, lymph nodes, liver, lungs, skin, soft tissues and bones with the aid of fine needle under negative pressure. Various techniques of cell block preparation lesion, and to classify neoplasms and other pathologic processes.[1,2,3,4] FNAC material may be analyzed with conventional smear (direct smear method), liquid-based cytology and preparation of cell block (CB), of which the latter two can further aid in the cytopathological diagnosis, facilitating ancillary studies.[5,6] Sometimes FNA does not yield sufficient information for precise diagnosis and the risk of false negative or indeterminate diagnosis exists. The study compared two methods of cell block preparation, plasma-thromboplastin method and collodion bag technique of cell block preparation
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